Melinda Ratini, DO, MS. As shown in Fig. You ggut take the steps to achieve an optimal weight.

CLA and gut health -

Int J Obes Lond ; 31 6 : —9. Curr Trends Biotechnol Pharm ; 12 2 : — Jin T, Jiang Z, Luan X, Qu Z, Guo F, Gao S, et al. Exogenous orexin-A microinjected into central nucleus of the amygdala modulates feeding and gastric motility in rats.

Front Neurosci. Quantifying size and number of adipocytes in adipose tissue. Methods Enzymol ; 93— Application of thin-layer chromatography-flame ionization detection TLC-FID to total lipid quantitation in mycolic-acid synthesizing Rhodococcus and Williamsia species. Int J Mol Sci ; 21 5 : Simultaneous determination and quantification of seven major phospholipid classes in human blood using normal-phase liquid chromatography coupled with electrospray mass spectrometry and the application in diabetes nephropathy.

J Chromatogr B Anal Technol Biomed Life Sci ; 1—2 : — Metabolites of conjugated isomers of linoleic acid CLA in the rat. Biochim Biophys Acta. Effect of orexin-A in the arcuate nucleus on cisplatin-induced gastric side effects in rats. Neurosci Res ; 53— Intestinal permeability in children with food allergy on specific elimination diets.

Pediatr Allergy Immunol ; 24 6 : — Bifidobacterium breve CCFM could ameliorate DSS-induced colitis in mice primarily via conjugated linoleic acid production and gut microbiota modulation.

J Funct Foods ; 61— Correlation analysis of intestinal redox state with the gut microbiota reveals the positive intervention of tea polyphenols on hyperlipidemia in high fat diet fed mice. J Agric Food Chem ; 67 26 : — J Food Sci ; 80 6 : R Increased iNOS activity is essential for intestinal epithelial tight junction dysfunction in endotoxemic mice.

Shock ; 21 3 : — Comparison of intestinal permeability, morphology, and ileal microbial communities of commercial hens housed in conventional cages and cage-free housing systems. Poult Sci ; 2 : — Pharmacological restoration of gut barrier function in stressed neonates partially reverses long-term alterations associated with maternal separation.

Psychopharmacology Berl. The gut microbiota: an emerging risk factor for cardiovascular and cerebrovascular disease. Eur J Immunol ; 48 4 : — Dietary trans, cisconjugated linoleic acid alters fatty acid metabolism and microbiota composition in mice.

Br J Nutr ; 5 : — Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids ; 32 2 : — The effect of conjugated linoleic acid isomers on fatty acid profiles of liver and adipose tissues and their conversion to isomers of and conjugated fatty acids in rats.

Lipids ; 36 6 : — Protective roles and mechanisms of Dendrobium officinal polysaccharides on secondary liver injury in acute colitis. Int J Biol Macromol ; Pt B : — Increased gut permeability and microbiota change associate with mesenteric fat inflammation and metabolic dysfunction in diet-induced obese mice.

PLoS One ; 7 3 : e Gut permeability is related to body weight, fatty liver disease, and insulin resistance in obese individuals undergoing weight reduction.

Am J Clin Nutr ; 1 : — Intestinal epithelial tight junctions and permeability can be rescued through the regulation of endoplasmic reticulum stress by amniotic fluid stem cells during necrotizing enterocolitis. FASEB J ; 35 1 : e Obese mice losing weight due to trans, cis conjugated linoleic acid supplementation or food restriction harbor distinct gut microbiota.

J Nutr ; 4 : — Saturated long-chain fatty acid-producing bacteria contribute to enhanced colonic motility in rats. Microbiome ; 6 1 : Inflamm Bowel Dis ; 24 2 : — Depletion of Blautia species in the microbiota of obese children relates to intestinal inflammation and metabolic phenotype worsening.

mSystems ; 5 2 : e— The genus alistipes: gut bacteria with emerging implications to inflammation, cancer, and mental health. Front Immunol ; The gut microbiota, bacterial metabolites and colorectal cancer.

Nat Rev Microbiol ; 12 10 : — Lactobacillus acidophilus alleviates type 2 diabetes by regulating hepatic glucose, lipid metabolism and gut microbiota in mice. Food Funct ; 10 9 : — Discrepant gut microbiota markers for the classification of obesity-related metabolic abnormalities. Sci Rep ; 9 1 : PDF HTML EPUB XML.

How to Cite. Gao S. More Citation Formats ACM ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation. Vol 66 Make a Submission. Most Read This Month Comparative validity of the ASSO - Food Frequency Questionnaire for the web-based assessment of food and nutrients intake in adolescents.

Fat and fatty acids — a scoping review for Nordic Nutrition Recommendations Vegetables, fruits, and berries — a scoping review for Nordic Nutrition Recommendations Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits.

The presence of this mucin-degrading bacterial species, which resides in the mucus layer, is associated to a healthy mucosa and is generally reduced in obese states [ 66 — 68 ]. Everard et al. muciniphila content in obese animals, and this is associated with an improvement of their metabolic profile.

Therefore, the increased caecal content of A. muciniphila found under CLA supplementation suggests that this compound was exerting a prebiotic action on this bacterial species too.

To our knowledge, this is the first evidence of a CLA-prebiotic effect favouring the specific growth of potentially beneficial bacterial species in the gut. muciniphila was not enough to allow a full recovery since these animals remain obese [ 35 ]. muciniphila content and have more significant effects on metabolic parameters.

This would fit with the lower induction of A. muciniphila found in the present study in comparison with others [ 69 , 71 ]. Overall, our data show that gastrointestinal tract is a first site for action of the bioactive isomers of CLA, able to modulate gastric responses as well as microbiota-host metabolism.

We cannot rule out the potential interplay between gastric environment and bacterial growth associated to food cues accompanying CLA intake. However, CLA induced the expression of genes encoding gastric proteins related with regulation of energy balance and exerted a prebiotic effect on selected bacterial species.

muciniphila , suggests CLA confers a prebiotic effect, which could contribute to a healthier metabolic profile. Further and thorough research of how specific dietary treatments influence specific physiological components, such as the gastrointestinal tract, will help elucidate their impact on specific conditions such as obesity, and develop efficient body weight management strategies.

We thank Sarah Laraichi Laboratory of Calorimetry and Materials, Faculty of Sciences, Abdelmalek Essaâdi University, Tétouan, Morocco for her help with the animal care and sample collection during her stay in our lab. Conceived and designed the experiments: PP AC AP FS.

Performed the experiments: AC PP. Analyzed the data: PP AC AP FS. Wrote the paper: FS AC AP. Browse Subject Areas? Click through the PLOS taxonomy to find articles in your field.

Article Authors Metrics Comments Media Coverage Reader Comments Figures. Abstract The gastrointestinal tract constitutes a physiological interface integrating nutrient and microbiota-host metabolism.

This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Data Availability: All relevant data are within the paper and its Supporting Information files.

Introduction Obesity is currently growing at an epidemic rate, considered a major health threat around the world, and resulting in an increased risk of diabetes mellitus type II, some types of cancer, fatty liver disease, hypertension, cardiovascular disease and increased mortality.

Growing research has shown that the isomers cis -9, trans CLA and trans , cis CLA in particular have a major role in the regulation of body weight and body fat in both animal [ 3 — 9 ] and human [ 10 — 13 ] studies , The present study was carried out to further characterise the effects of CLA on body weight management, by addressing certain aspects that to our knowledge have not been studied before.

Sacrifice and sample collection Sacrifice of all animals was carried out within the animal facilities, at the beginning of the light cycle and after 10h of food deprivation. Download: PPT. Table 1. Nucleotide sequences of primers used for qPCR amplification in mouse stomach.

Bacterial profiling by qPCR Total bacterial DNA was extracted from approximately 50 mg of caecal samples using the E. Table 2. Sequence of primers used for bacterial profiling in caecum content. Statistical analysis Data are presented as means ± SEM.

Results CLA supplementation modulates the expression of regulatory proteins in the stomach Expression of proteins associated to energy metabolism and regulation of food intake was determined in mouse stomach.

Fig 1. Effect of CLA supplementation on mRNA expression and protein levels of gastric proteins in mice. Caecum microbiota is modulated by CLA To further look into the effect of CLA, caecum content of mice was analysed in order to determine bacterial species potentially associated to obesity and energy metabolism.

Fig 2. DNA levels of representative bacterial species in mice caecum are altered by diet. Caecum microbiota correlates with body weight and body fat We next tested the hypothesis that abundance of specific bacterial species in mouse caecum contents could be associated to modulation of body weight and body fat.

Table 3. Correlations between bacterial species in caecum contents with body weight and body fat of mice. Discussion The present study provides evidence that CLA supplementation under a HF diet has a noticeable effect on particular sites of the gastrointestinal tract in mouse, by increasing gastric protein expression and by promoting a prebiotic effect on gut microbiota.

Supporting Information. S1 Table. Detailed composition of diets. Composition of normal and high fat diets used throughout the experiment. s DOCX.

Acknowledgments We thank Sarah Laraichi Laboratory of Calorimetry and Materials, Faculty of Sciences, Abdelmalek Essaâdi University, Tétouan, Morocco for her help with the animal care and sample collection during her stay in our lab.

Author Contributions Conceived and designed the experiments: PP AC AP FS. References 1. Swinburn BA, Sacks G, Hall KD, McPherson K, Finegood DT, Moodie ML, et al. The global obesity pandemic: shaped by global drivers and local environments.

Trigueros L, Pena S, Ugidos AV, Sayas-Barbera E, Perez-Alvarez JA, Sendra E. Food ingredients as anti-obesity agents: a review. Crit Rev Food Sci Nutr. Park Y, Albright KJ, Liu W, Storkson JM, Cook ME, Pariza MW.

Effect of conjugated linoleic acid on body composition in mice. Takahashi Y, Kushiro M, Shinohara K, Ide T. Dietary conjugated linoleic acid reduces body fat mass and affects gene expression of proteins regulating energy metabolism in mice.

Comp Biochem Physiol B Biochem Mol Biol. West DB, Blohm FY, Truett AA, DeLany JP. J Nutr. West DB, Delany JP, Camet PM, Blohm F, Truett AA, Scimeca J. Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse.

Am J Physiol. Zabala A, Fernandez-Quintela A, Macarulla MT, Simon E, Rodriguez VM, Navarro V, et al. Effects of conjugated linoleic acid on skeletal muscle triacylglycerol metabolism in hamsters. Kanaya N, Chen S. Nutr Res. pmid; PubMed Central PMCID: PMC Park Y, Albright KJ, Storkson JM, Liu W, Pariza MW.

Conjugated linoleic acid CLA prevents body fat accumulation and weight gain in an animal model. J Food Sci. Smedman A, Vessby B.

Conjugated linoleic acid supplementation in humans—metabolic effects. Blankson H, Stakkestad JA, Fagertun H, Thom E, Wadstein J, Gudmundsen O.

Conjugated linoleic acid reduces body fat mass in overweight and obese humans. Thom E, Wadstein J, Gudmundsen O. Conjugated linoleic acid reduces body fat in healthy exercising humans. J Int Med Res. Gaullier JM, Halse J, Hoivik HO, Hoye K, Syvertsen C, Nurminiemi M, et al.

Six months supplementation with conjugated linoleic acid induces regional-specific fat mass decreases in overweight and obese. Br J Nutr. Martins dos Santos V, Muller M, de Vos WM. Systems biology of the gut: the interplay of food, microbiota and host at the mucosal interface.

Curr Opin Biotechnol. Hernandez E, Bargiela R, Diez MS, Friedrichs A, Perez-Cobas AE, Gosalbes MJ, et al. Functional consequences of microbial shifts in the human gastrointestinal tract linked to antibiotic treatment and obesity. Gut Microbes. Erdmann J, Lippl F, Schusdziarra V.

Differential effect of protein and fat on plasma ghrelin levels in man. Regul Pept. Greenman Y, Golani N, Gilad S, Yaron M, Limor R, Stern N.

Ghrelin secretion is modulated in a nutrient- and gender-specific manner. Clin Endocrinol Oxf. Wadden D, Cahill F, Amini P, Randell E, Vasdev S, Yi Y, et al. Serum acylated ghrelin concentrations in response to short-term overfeeding in normal weight, overweight, and obese men.

PLoS One. Sun B, Purcell RH, Terrillion CE, Yan J, Moran TH, Tamashiro KL. Maternal high-fat diet during gestation or suckling differentially affects offspring leptin sensitivity and obesity. Zhao Z, Sakata I, Okubo Y, Koike K, Kangawa K, Sakai T. Gastric leptin, but not estrogen and somatostatin, contributes to the elevation of ghrelin mRNA expression level in fasted rats.

J Endocrinol. Scarpace PJ, Zhang Y. Leptin resistance: a prediposing factor for diet-induced obesity. Am J Physiol Regul Integr Comp Physiol. Backhed F, Ding H, Wang T, Hooper LV, Koh GY, Nagy A, et al. The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci U S A.

Cani PD, Delzenne NM. Interplay between obesity and associated metabolic disorders: new insights into the gut microbiota.

Curr Opin Pharmacol. Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER, Gordon JI. An obesity-associated gut microbiome with increased capacity for energy harvest. Murphy EF, Cotter PD, Healy S, Marques TM, O'Sullivan O, Fouhy F, et al. Composition and energy harvesting capacity of the gut microbiota: relationship to diet, obesity and time in mouse models.

Ley RE, Backhed F, Turnbaugh P, Lozupone CA, Knight RD, Gordon JI. Obesity alters gut microbial ecology. Backhed F, Fraser CM, Ringel Y, Sanders ME, Sartor RB, Sherman PM, et al. Defining a healthy human gut microbiome: current concepts, future directions, and clinical applications.

Cell Host Microbe. Le Chatelier E, Nielsen T, Qin J, Prifti E, Hildebrand F, Falony G, et al. Richness of human gut microbiome correlates with metabolic markers. Cotillard A, Kennedy SP, Kong LC, Prifti E, Pons N, Le Chatelier E, et al. Dietary intervention impact on gut microbial gene richness.

David LA, Maurice CF, Carmody RN, Gootenberg DB, Button JE, Wolfe BE, et al. Diet rapidly and reproducibly alters the human gut microbiome.

Zhang C, Zhang M, Wang S, Han R, Cao Y, Hua W, et al. Interactions between gut microbiota, host genetics and diet relevant to development of metabolic syndromes in mice. Isme J. Zhang C, Zhang M, Pang X, Zhao Y, Wang L, Zhao L.

Structural resilience of the gut microbiota in adult mice under high-fat dietary perturbations. Parks BW, Nam E, Org E, Kostem E, Norheim F, Hui ST, et al.

Genetic control of obesity and gut microbiota composition in response to high-fat, high-sucrose diet in mice. Cell Metab. Brownawell AM, Caers W, Gibson GR, Kendall CW, Lewis KD, Ringel Y, et al. Prebiotics and the health benefits of fiber: current regulatory status, future research, and goals.

Laraichi S, Parra P, Zamanillo R, El Amarti A, Palou A, Serra F. Dietary supplementation of calcium may counteract obesity in mice mediated by changes in plasma fatty acids.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. Sanchez J, Oliver P, Palou A, Pico C. The inhibition of gastric ghrelin production by food intake in rats is dependent on the type of macronutrient.

Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. Parnell JA, Reimer RA. Prebiotic fibres dose-dependently increase satiety hormones and alter Bacteroidetes and Firmicutes in lean and obese JCR:LA-cp rats.

Sonoyama K, Ogasawara T, Goto H, Yoshida T, Takemura N, Fujiwara R, et al. Neary MT, Batterham RL. Gut hormones: implications for the treatment of obesity. Pharmacol Ther. Cammisotto P, Bendayan M. A review on gastric leptin: the exocrine secretion of a gastric hormone.

Anat Cell Biol. Date Y, Kojima M, Hosoda H, Sawaguchi A, Mondal MS, Suganuma T, et al. Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans.

Sanchez J, Priego T, Oliver P, Palou A, Pico C. The intake of a hyperlipidic diet stimulates the gastric leptin signalling pathway in female rats.

Genes Nutr. Cinti S, de Matteis R, Ceresi E, Pico C, Oliver J, Oliver P, et al. Leptin in the human stomach. Oliver P, Pico C, De Matteis R, Cinti S, Palou A. Perinatal expression of leptin in rat stomach. Dev Dyn. Palou A, Pico C. Leptin intake during lactation prevents obesity and affects food intake and food preferences in later life.

Bednarek MA, Feighner SD, Pong SS, McKee KK, Hreniuk DL, Silva MV, et al. Structure-function studies on the new growth hormone-releasing peptide, ghrelin: minimal sequence of ghrelin necessary for activation of growth hormone secretagogue receptor 1a.

J Med Chem. Sanchez J, Oliver P, Pico C, Palou A. Diurnal rhythms of leptin and ghrelin in the systemic circulation and in the gastric mucosa are related to food intake in rats.

Pflugers Arch. McKee KK, Tan CP, Palyha OC, Liu J, Feighner SD, Hreniuk DL, et al.

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